Ruth Alonso1, Diana Pisa1, Ana Isabel Marina1, Esperanza Morato1, Alberto Rábano2, Izaskun Rodal2 a
- Centro de Biología Molecular “Severo Ochoa”. c/Nicolás Cabrera, 1. Universidad Autónoma de
Madrid. Cantoblanco. 28049 Madr Spain.
- Department of Neuropathology and Tissue Bank, Unidad de Investigación Proyecto Alzheimer,
Fundación CIEN, Instituto de Salu Carlos III, Madrid. Spain.
Among neurogenerative diseases, amyotrophic lateral sclerosis (ALS) is a fatal illness characterized by a progressive motor neuron dysfunction in the motor cortex, brainstem and spinal
cord. ALS is the most common form of motor neuron disease; yet, to date, the exact etiology of ALS remains unknown. In the present work, we have explored the possibility of fungal infection in
cerebrospinal fluid (CSF) and in brain tissue from ALS patients. Fungal antigens, as well as DNA from several fungi, were detected in CSF from ALS patients. Additionally, examination of brain
sections from the frontal cortex of ALS patients revealed the existence of immunopositive fungal antigens comprising punctate bodies in the cytoplasm of some neurons. Fungal DNA was also detected in brain tissue using PCR analysis, uncovering the presence of several fungal species. Finally, pro- teomic analyses of brain tissue demonstrated the occurrence of several fungal peptides.
Collectively, our observations provide compelling evidence of fungal infection in the ALS patients analyzed, suggesting that this infection may play a part in the etiology of the disease
or may constitute a risk factor for these patients.
International Journal of Biological Sciences
2015; 11(5): 546-558. doi: 10.7150/ijbs.1